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human brca cell line mda mb 231  (ATCC)


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    ATCC human brca cell line mda mb 231
    Human Brca Cell Line Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 24163 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 24163 article reviews
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    MEP promote the malignant biological behavior of MCF-7 (A) qRT-PCR data showing CDK1, EZH2, E2F1, and CCNE1 mRNA expression. (B) Western blots showing CDK1, EZH2, E2F1, and CCNE1 expression. (C) Macrographs of colony formation and quantitative analysis. (D) Flow cytometry showing cell cycle distribution and cell percentages in different phases. (E) Wound healing assay with quantitative analysis was performed to assess the migratory capacity. Scale bar 650 μm. (F) Representative cell migration images and migration index. Scale bar 275 μm. (G) Phalloidin staining was employed to evaluate F-actin reorganization. Scale bar 25 μm. (H) Western blots showing E-cadherin, Vimentin, MMP2, and MMP9 expression. Data are presented as the mean ± standard deviation ( n = 3), * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001 when compared with control cells.

    Journal: ACS Omega

    Article Title: Deciphering the Effect of Methyl 4‑Hydroxybenzoate on Breast Cancer by Bioinformatics and Experiments

    doi: 10.1021/acsomega.5c11680

    Figure Lengend Snippet: MEP promote the malignant biological behavior of MCF-7 (A) qRT-PCR data showing CDK1, EZH2, E2F1, and CCNE1 mRNA expression. (B) Western blots showing CDK1, EZH2, E2F1, and CCNE1 expression. (C) Macrographs of colony formation and quantitative analysis. (D) Flow cytometry showing cell cycle distribution and cell percentages in different phases. (E) Wound healing assay with quantitative analysis was performed to assess the migratory capacity. Scale bar 650 μm. (F) Representative cell migration images and migration index. Scale bar 275 μm. (G) Phalloidin staining was employed to evaluate F-actin reorganization. Scale bar 25 μm. (H) Western blots showing E-cadherin, Vimentin, MMP2, and MMP9 expression. Data are presented as the mean ± standard deviation ( n = 3), * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001 when compared with control cells.

    Article Snippet: The human BRCA cell line MCF-7 was obtained from ATCC and cultured in minimum essential medium (Gibco, Carlsbad, CA, USA) in an incubator at 37 °C and 5% CO 2 .

    Techniques: Quantitative RT-PCR, Expressing, Western Blot, Flow Cytometry, Wound Healing Assay, Migration, Staining, Standard Deviation, Control